Archives of Head and Neck Surgery
https://archivesheadnecksurgery.com/article/doi/10.4322/ahns.2018.0874
Archives of Head and Neck Surgery
Original Article Characterization of thyroid tissue using infrared spectroscopy

Characterization of thyroid tissue using infrared spectroscopy

Luiz Flávio de Azevedo Villela, Luiz Carlos Conti de Freitas, Mariana Brandão, Luciano Bachmann, Ricardo Iwakura

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Abstract

Introduction: In recent years, the number of surgical procedures involving the thyroid gland has increased worldwide; however, no increase in mortality rate has been observed. The Fourier Transform Infrared (FTIR) spectroscopy technique presents evidence in the characterization of multiple tissues, such as the thyroid gland, with the advantage of being rapid and preserving the tissue analyzed. Objectives: Characterize both healthy and pathological thyroid tissues by FTIR spectroscopy. Methods: Patients were selected at the Head and Neck Surgery Service of the Hospital das Clínicas, Ribeirão Preto Medical School, University of Sao Paulo - USP, Ribeirão Preto-SP, Brazil, from 2014 to 2015. The sample consisted of 44 patients with reference for thyroidectomy. The analysis was performed by defining the areas of each band using the OriginPro 8.6.0 software. The band was then normalized to 1240 cm-1. The mean area was calculated using the Student’s t-test with p<0.05. After calculation of the means, the second-order derivative of the spectrum was evaluated to show the positions of each absorption band. Results: The infrared spectrum of each piece was obtained and expressed as a function of absorbance and wave numbers in the mean IR (4000-900 cm-1). The present study demonstrated that, in the analysis of thyroid tissue by FTIR spectroscopy, it is possible to differentiate benign nodules from healthy tissue with significant difference in the area of the B-band between healthy tissue and goiter, which corresponds to 1452.90 cm-1 in healthy tissue (proteins and lipids) and 1069.80 cm-1 in goiter (DNA), as well as significant difference in width between normal thyroid tissue and carcinoma of the C band. Conclusions: FTIR spectroscopy is able to differentiate pathologically altered thyroid tissues from the thyroid gland compared with findings in healthy thyroid tissues. In patients with benign nodular disease of the thyroid gland, it is possible to differentiate healthy goiter tissue with statistical significance, as well as malignant nodules from healthy tissue through FTIR spectroscopy.

Keywords

thyroid neoplasms; spectroscopy; fourier transform infrared; goiter

References

1. Instituto Nacional de Câncer. Coordenação de Prevenção e Vigilância. Estimativa 2016: incidência de câncer no Brasil [Internet]. Rio de Janeiro: INCA; 2015. 122 p. [cited 2016 Jul 20]. Available from: http://www.inca.gov.br/estimativa/2016/estimativa-2016-v11.pdf

2. Lira RB, Carvalho GB, Gonçalves J Fo, Kowalski LP. Evolution in the profile of thyroid cancer cases treated in an oncology reference service: what changed in the last 20 years. Rev Col Bras Cir. 2014;41(5):320-4. http://dx.doi.org/10.1590/0100- 69912014005004. PMid:25467095.

3. Kitahara CM, Sosa JA. The changing incidence of thyroid cancer. Nat Rev Endocrinol. 2016;12(11):646-53. http://dx.doi.org/10.1038/nrendo.2016.110. PMid:27418023.

4. Ali K, Lu Y, Christensen C, May T, Hyett C, Griebel R, Fourney D, Meguro K, Resch L, Sharma RK. Fourier transform Infrared spectromicroscopy and hierarchical cluster analysis of human meningiomas. Int J Mol Med. 2008;21(3):297-301. PMid:18288376.

5. Carneiro PC, Domingues RB. Punção aspirativa com agulha fina em tireoide e paratireoide. In: Volpi E, Steck JH, editors. Cirurgia da tireoide e da paratireoide. São Paulo: AC Farmacêutica; 2013. p. 79-81.

6. Biomarkers Definitions Working Group. Biomarkers and surrogate endpoints: preffered definitions and conceptual framework. Clin Pharmacol Ther. 2001;69(3):89-95. http://dx.doi.org/10.1067/mcp.2001.113989. PMid:11240971.

7. Montoro JR, Mamede RC, Neder Serafini L, Saggioro FP, Figueiredo DL, Silva WA Jr, Jungbluth AA, Spagnoli GC, Zago MA. Expression of cancer-testis antigens MAGE-4 and MAGE-C1 in oral squamous cell carcinoma. Head Neck. 2011 PMid:22083937.

8. Mostaço-Guidolin LB. Caracterização bioquímica de células sadias e neoplásicas através de espectroscopia vibracional [dissertação]. Ribeirão Preto: Faculdade de Filosofia, Ciências e Letras de Ribeirão Preto, Universidade de São Paulo; 2009.

9. Fundação Oswaldo Cruz. Instituto de Comunicação e Informação Científica e Tecnológica em Saúde (PROQUALIS). Rio de Janeiro: Fiocruz; 2017 [cited 2017 Jan 25]. Available from: http://proqualis.net/glossrio/overtreatment

10. Vaccarella S, Franceschi S, Bray F, Wild CP, Plummer M, Dal Maso L. Worldwide thyroid cancer epidemic? The increased impact of overdiagnosis. N Engl J Med. 2016;375(7):614-7. http://dx.doi.org/10.1056/NEJMp1604412. PMid:27532827.

11. Zhang X, Xu Y, Zhang Y, Wang L, Hou C, Zhou X, Ling X, Xu Z. Intraoperative detection of thyroid carcinoma by Fourier transform infrared spectrometry. J Surg Res. 2011;171(2):650-6. http://dx.doi.org/10.1016/j.jss.2010.05.031. PMid:20828740.

12. Wu M, Zhang W, Tian P, Ling X, Xu Z. Intraoperative diagnosis of thyroid diseases by Fourier transform Infrared spectroscopy based on support vector machine. Int J Clin Exp Med. 2016;9(2):2351-8.

13. Andrus PGL, Strickland RD. Cancer grading by Fourier transform infrared spectroscopy. Biospectroscopy. 1998;4(1):37-46. http://dx.doi.org/10.1002/(SICI)1520-6343(1998)4:1<37::AID-BSPY4>3.0.CO;2-P. PMid:9547013.

14. Rosário PW, Ward LS, Carvalho GA, Graf H, Maciel RM, Maciel LM, Maia AL, Vaisman M. Nódulo tireoidiano e câncer diferenciado de tireoide: atualização do consenso brasileiro. Arq Bras Endocrinol Metabol. 2013;57(4):240-64. PMid:23828432.

15. McWade MA, Paras C, White LM, Phay JE, Mahadevan-Jansen A, Broome JT. A novel optical approach to intraoperative detection of parathyroid glands. Surgery. 2013;154(6):1371-7. http://dx.doi.org/10.1016/j.surg.2013.06.046. PMid:24238054.

16. Liu Y, Xu Y, Liu Y, Zhang Y, Wang D, Xiu D, Xu Z, Zhou X, Wu J, Ling X. Detection of cervical metastatic lymph nodes in papillary thyroid carcinoma by Fourier transform infrared spectroscopy. Br J Surg. 2011;98(3):380-4. http://dx.doi.org/10.1002/bjs.7330. PMid:21254012.

17. Shaw RA, Guijon FB, Paraskevas M, Ying SL, Mantsch HH. Infrared spectroscopy of exfoliated cervical cell specimens: Proceed with caution. Anal Quant Cytol Histol. 1999;21(4):292-302. PMid:10560506.

18. Sarkar A, Sengupta S, Mukherjee A, Chatterjee J. Fourier transform ifra-red spectroscopic signatures for lung cells epithelial mesenchymal transition: A preliminar report. Spectrochim Acta A Mol Biomol Spectrosc. 2017;173:809-16. http://dx.doi.org/10.1016/j.saa.2016.10.019. PMid:27810772.

19. Theophilou G, Lima KM, Briggs M, Martin-Hirsch PL, Stringfellow HF, Martin FL. A biospectroscopic analysis of human prostate tissue obtained from different time periods points to a trans-generational alteration in spectral phenotype. Sci Rep. 2015;5(1):13465. http://dx.doi.org/10.1038/srep13465. PMid:26310632.

20. Dudala J, Białas MB, Szczerbowska-Boruchowska M, Bereza-Buziak M, Budzynski A, Hubalewska-Dydejczyk A, Kolodziej M, Pedziwiatr M, Lankosz M. Investigation of biochemical composition of adrenal gland tumors by means of FTIR. Pol J Pathol. 2016;67(1):60-8. http://dx.doi.org/10.5114/pjp.2016.59221. PMid:27179276.

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