Archives of Head and Neck Surgery
https://archivesheadnecksurgery.com/article/doi/10.4322/ahns.2019.0009
Archives of Head and Neck Surgery
Original Article Carcinogenesis, tumor biology and tumor markers

Hox genes and laryngeal carcinogenesis

Rafaela de Barros e Lima Bueno, Anelisa Ramão, Daniel Guariz Pinheiro, Cleidson Padua Alves, Vinicius Kannen, Achim Jungbluth, Luiza Ferreira de Araújo, Bruna Rodrigues Muys, Aline Simoneti Fonseca, Jessica Rodrigues Plaça, Bárbara Mendes Paz Chao, Felipe da Silva Figueiredo, Rodrigo Alexandre Panepucci, Luciano Neder, Fabiano Saggioro, Rui Celso Martins Mamede, David Livingstone Alves Figueiredo, Wilson Araújo Silva Junior

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Abstract

Introduction: Larynx squamous cell carcinoma (LSCC) is a very aggressive type of cancer. Despite the meaningful advances in the understanding and treatment of this cancer, the prognosis of LSCC patients has not improved recently. Objective: In the present study, we sought to better understand the mechanisms subjacent to LSCC development. Methods: Thirty-two tumor samples were collected from patients submitted to LSCC resection. The samples were submitted to cDNA microarray analysis to identify LSCC targets. We also applied bioinformatics approach and performed functional testing, using human cell lines from head and neck. We assessed the feasibility, cell proliferation and cell migration after the selected gene silencing. Results: Eight members from the homeobox (HOX) gene family were identified as super expressed in LSCC when compared to the samples or normal tissues of the larynx, which was validated with RT-PCR quantitative analysis. Clinical data correlation with the genic expression revealed that the genes HOXC8 and HOXD11 genes were associated with the level of differentiation of regional tumors and lymph nodes metastasis, respectively. Besides, siRNA testing confirmed that HOXC8, HOXD10, and HOXD11 genes could be critical for the proliferation and cell migration. Conclusion: According to our discoveries, several HOX genes members were super expressed in LSCC samples and seem to be necessary in biological processes involved in tumor development. This suggests that HOX genes can play a critical role in the physiopathology of LSCC tumors.

Keywords

larynx squamous cell carcinoma; gene regulation; HOX genes; cell migration.

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