Parotid matastasis of clear-cell renal cell carcinoma (ccRCC): a case report
Mariana Brito Martire, Luís Flávio Villena, Jairo Alves de Sousa Junior, José Raphael Moura Campos Montoro, Silvio Antônio Bertacchi Uvo
Introduction: Malignant neoplasm of the mouth is a very common disease, being the sixth most common cancer location worldwide. Objective: This study was conducted with the aim of evaluating the applicability and the relationship between cell damage intensity and Oral cavity squamous cell carcinoma (OCSCC) clinical stages. Methods: A total of 44 individuals, 24 oral cancer patients and 20 healthy volunteers participated in this study. The exfoliated cells from the oral cavity were collected using a wooden spatula. All samples were analyzed by comet assay, micronucleus assay and cell death assay. Results: Our results indicate that the diagnosis of OCSCC increases the frequency of genotoxic damage (comet assay – 3.21x; micronucleus assay – 3.93x). However, it was not possible to establish a correlation between frequency of DNA damage and disease staging. The cell death assay was not effective for the proposed biomonitoring. Given these findings, we consider the comet and micronucleus assays to be adequate for biomonitoring DNA damage. Conclusion: With the current design, we could not demonstrate the applicability of these assays to correlate the intensity of DNA/cell damage and the clinical stages of OCSCC. Nevertheless, further studies need to be performed to enhance sample size and increase the statistical power of our findings
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